Contact information

Principle Investigator:

Diasynou Fioravante

 

Center for Neuroscience

Univ. of California Davis

1544 Newton Court

Davis CA 95618

 

Lab: (530) 754 7382

Office: (530) 752 1887

 

email:

dfioravante@ucdavis.edu

From synapses to microcircuits

Cerebellar projections

Cerebellar projections

Neurons in the cerebellar nuclei (bright yellow) communicate with subcortical brain areas through numerous axonal projections, the functions of which we investigate in the lab.

Cerebellum

Cerebellum

We use targeted injections of viruses to achieve localized expression of different proteins. Here, Purkinje neurons within specific cerebellar lobules express channelrhodopsin-GFP (green). Note the axons coursing through the white matter on their way to deep cerebellar nuclei. Fioravante Lab, unpublished.

Transgenic lines

Transgenic lines

For the study of microcircuits, we use various mouse lines to achieve cell-type specific expression of opsins. Here, channelrhodopsin-mCherry is expressed in the cerebellum of an L7-Cre mouse. Fioravante, unpublished.

Purkinje neurons

Purkinje neurons

The sole output of the cerebellar cortex, Purkinje neurons are labeled with antibodies against protein kinase C. From Fioravante et al., 2012.

Calyces of Held

Calyces of Held

The calyx of Held synapse in the auditory brainstem is a powerful model system for the study of synaptic transmission. Here, calyces express a mutated form of protein kinase C (green) and are stained for vGlut1 (red).

Calyces of Held

Calyces of Held

For 2-photon calcium imaging at mature synapses, calcium dyes are bulk-loaded in the presynaptic terminals together with a structural dye (Alexa 594, red). Fioravante, unpublished.

Neocortex

Neocortex

We can manipulate gene expression in the cortex and hippocampus through in utero electroporation. Here, using this technique, green fluorescence protein is expressed in different cortical layers. Thanawala and Fioravante, unpublished.